Anti-TRAF1; clone 1F3

Analysis Note

ControlB95-8cell lysate

Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in certain cell lines (Siegler, G., et al. (2003). J Clin Pathol: Mol Pathol. 56:156-161.).
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in PBMCs and in certain T cells from HIV-infected individuals (Wang, C., et al. (2011). JEM.1-15.).

Research Sub CategoryApoptosis - Additional

Anti-TRAF1, clone 1F3 detects levels of TRAF1 proteins & has been published & validated for use in WB, IHC & Flow.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TNF receptor-associated factor 1 (UniProt: Q13077; also known as Epstein-Barr virus-induced protein 6, TRAF1) is encoded by the TRAF1 (also known as EB16) gene (Gene ID: 7185) in human. TRAF1 is homodimeric pro-survival adapter molecule that regulates the activation of NF-kB and JNK. It can also exist as a heterotrimer with TRAF2. It plays a role in the regulation of cell survival and apoptosis. The heterotrimer form is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. The C-terminal region of TRAF1 contains the TRAF domain (aa 266-412) that binds to receptor cytoplasmic domains. It also has a coiled coil domain (aa 182-264) that mediates its homo- and heterotrimerization. TRAF1 is also reported to essential for memory T cell survival. TRAF1 protein level is significantly reduced in HIV-specific CD8 T cells with progression of infection. Its levels can increase following treatment with IL-7. Hence, TRAF1 levels could serve as a useful parameter in monitoring effectiveness of anti-HIV therapies. (Ref.: Wang, C., et al. (2018). J. Immunol. 200(2); 558-564).

Immunogen

GST-tagged recombinant protein corresponding to human TRAF1.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in B95-8 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected TRAF1 in 10 µg of B95-8cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~46 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~33 kDa